L.
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Limitations
of the Procedure |
To ensure accurate and reliable results, every effort
should be made to perform the CAPE
Technologies High Performance Dioxin/Furan
Immunoassay Kit at temperatures between 20°C (68°F)
and 27°C (81°F).
The distribution of PCDD/Fs can be extremely heterogeneous.
Adequate sample number and distribution are the responsibility
of the analyst.
Samples analyzed by EIA must contain either immunoassay
compatible internal standards or no internal standards.
Consult Technical Note TN-001
and Calculation Module
D for discussion and other assistance.
The CAPE Technologies
High Performance Dioxin/Furan Immunoassay Kit is designed
for screening of samples according to their TEQ by responding
to the toxic PCDD/F congeners in approximate correlation
with their TEFs. Quantitative interpretation of data may
be possible for advanced users in certain situations.
Consult Technical Note TN-004
for discussion of this topic.
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M.
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Storage |
* |
Store all test kit components at 2°C to
6°C (36 °F to 43°F) when not in use. |
* |
Do not expose test kit components to temperatures
greater than 37°C (99°F). Storage at ambient temperature
(20°C to 27°C or 68°F to 81°F) on the day
of use is acceptable. Prolonged exposure (many days) or repeated
exposure to ambient temperatures may cause a loss of reagent
(especially conjugate) activity, resulting in decreased OD
values for all tubes. |
* |
Do not freeze test kit components. Kits
which have been frozen must not be used. |
* |
If desiccant in tube bag is not blue,
do not use kit; contact CAPE
Technologies. |
* |
Do not use test kit components after the expiration
date printed on the kit box label.
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N.
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Quality Assurance |
* |
Samples which appear milky during the first
EIA incubation probably contain more oil than can be tolerated
by the test. These samples should be diluted or cleaned more,
then analyzed again by EIA. |
* |
Response values for 2,3,7,8-TCDD standards typically
should be in the ranges given in
Table
1. |
* |
If a blue color does not develop in the negative
control test tube within 15 minutes after adding the substrate
solution, the test is invalid and must be repeated. If the
problem persists, contact CAPE Technologies. |
* |
The antibody used in this immunoassay
recognizes PCDD/F congeners based on structure, not mass.
Therefore, conventional stable isotope labeled internal standards
are detected as native material. Typical levels of conventional
stable isotope labeled internal standards can not be used
with this EIA. Please consult Technical Note TN-001
for recommendations on the use of immunoassay compatible mass
labeled internal standards. Contact CAPE
Technologies if additional assistance is required
regarding these issues. Immunoassay compatible internal standards
are available from Wellington Laboratories (Guelph, Ontario,
Canada). |
* |
Replication, check samples, standard reference
materials, and other QA samples and methods can and should
be used with this kit, with the exception of conventional
stable isotope labeled internal standards (as noted in the
above paragraph). The Starter Kit DF1-ST
includes two toluene check samples which should be used to
determine if the solvent exchange portion of the sample preparation
has been performed properly. Consult Wellington
Laboratories to obtain additional QA
materials.
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O.
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References |
Additional data related to the CAPE
Technologies High Performance Dioxin/Furan
Immunoassay Kit are available from CAPE
Technologies. Application Notes describe procedures
for analysis of a variety of matrices, TEQ levels, and
sample preparation methods. Technical Notes provide discussion
and recommendations pertaining to important technical
issues. Technical References are papers from technical
journals or other sources which provide extensive background
information about selected topics relevant to the immunoassay
analysis of PCDD/Fs. A list of these items is available
from CAPE Technologies.
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P.
|
General Limited
Warranty |
CAPE Technologies,
LLC ("CAPE Technologies")
warrants the products manufactured by it against defects
in materials and workmanship when used in accordance with
the applicable instructions for a period not to extend
beyond the expiration date printed on the product. CAPE
Technologies MAKES NO OTHER WARRANTY, EXPRESSED
OR IMPLIED. THERE IS NO WARRANTY OF MERCHANTABILITY OR
FITNESS FOR A PARTICULAR PURPOSE. The warranty provided
herein and the data, specifications and descriptions of
CAPE Technologies
products appearing in CAPE Technologies'
published catalogues and product literature may not be
altered except by express written agreement signed by
an officer of CAPE Technologies.
Representations, oral or written, which are inconsistent
with this warranty or such publications are not authorized
and if given, should not be relied upon.
In the event of a breach of the foregoing warranty, CAPE
Technologies' sole obligation shall be to repair
or replace, at its option, any product or part thereof
that proves defective in materials or workmanship within
the warranty period, provided the customer notifies CAPE
Technologies promptly of any such defect. The
exclusive remedy provided herein shall not be deemed to
have failed of its essential purpose so long as CAPE
Technologies is willing and able to repair
or replace any nonconforming CAPE
Technologies product or part. CAPE
Technologies shall not be liable for consequential,
incidental, special or any other indirect damages resulting
from economic loss or property damage sustained by any
customer from the use of its products.
Copyright 1998, CAPE Technologies,
LLC.
CAPE Technologies
is a trademark of CAPE Technologies,
LLC.
Eppendorf, Repeater and Repeater Plus are trademarks of
Eppendorf-Netheler-Hinz GmbH.
Drummond is a trademark of Drummond Scientific Co.
Artel is a trademark of Artel, Inc.
Microsoft and Excel are trademarks of Microsoft Corporation.
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