The CAPE Technologies High Performance Dioxin/Furan Immunoassay Kit is designed to provide screening (semiquantitative) results at predetermined or user selected levels. Advanced users may wish to produce quantitative results in certain situations. Development of quantitative data using the DF1 kit is possible, but requires care and experience. The highest probability of success is for analysis of many samples from one source. Experience and careful use of quality assurance (QA) samples will increase the probability of success.

CAPE Technologies provides the items necessary for quantitative analysis by EIA. Standards of 2,3,7,8-TCDD in methanol are available in the DF1-ST Starter Kit or separately from Wellington Laboratories. Use of all 4 of these standards in one EIA run will define the standard curve described in section D (Table 1) of the kit insert IN-DF1. The required calculations can be performed using Calculation Module C, a Microsoft Excel workbook, from CAPE Technologies. Module C is available for download or by email request:
(cape-tech@ceemaine.org).

Because of the intrinsically sigmoid shape of immunoassay response curves, Module C uses an iterative non-linear curve fitting procedure based on the same four parameter equation which is the basis for most commercial immunoassay data analysis software. Module C calculates the best fit standard curve and concentrations of unknowns. Full instructions are included in Calculation Module C. A more complete description of quantitative immunoassay analysis and its application to dioxin EIA is being developed.