Materials List for DF1 Starter Kit (DF1-ST)
Last Revised: 12/4/98

(This kit is designed for the first time user. It contains the EIA tubes and reagents of the DF1-60 kit plus selected items needed for start-up.)

Contents of This Materials List

A.
Materials included in DF1-ST
(shipped from CAPE Technologies)
B.

Materials included in DF1-ST
(shipped from Wellington Labs)

C. Calculation Modules Available

A.

Materials included in DF1-ST
(shipped from CAPE Technologies)

* Kit insert (IN-DF1)
* Brief Summary of Immunoassay Protocol (PS-DF1)
* 80 Anti-Dioxin "Antibody Coated Tubes" (4 bags of 20 tubes with indicating desiccants)
* 1 bottle "Competitor-HRP Conjugate" (50 mL/bottle)
* 1 bottle "HRP Substrate Solution" (50 mL/bottle)
* 1 bottle "Stop Solution" (50 mL/bottle)
* 1 vial of 0.5 mL of neat Triton X-100
* 1 test tube rack with assembly instruction sheet
* 1 bag of uncoated tubes (rims marked black) for pipetting practice and photometer blanking


B.
Materials included in DF1-ST
(shipped from Wellington Labs)
* one set of negative control (methanol + 200 ppb Triton X-100) and 4 dioxin standards (0.32, 1.0, 3.2, and 10 ng/mL 2,3,7,8-TCDD in negative control) in ampoules (0.25 mL each)
* DF1-CS1 (check sample in toluene; 500 µL of 180 pg/mL 2,3,7,8-TCDD in methanol/Triton)
* DF1-CS2 (check sample in toluene; 500 µL of 600 pg/mL 2,3,7,8-TCDD in methanol/Triton)
Additional sets of standards and check samples must be ordered directly from Wellington Laboratories (info@well-labs.com) or their distributor, TerraChem (shallstrom@cwix.com).

 

C.
Calculation Modules Available
for Download
  Calculation Modules are Excel 5.0 workbooks designed for certain calculation tasks associated with the use of the DF1 Kit (description and instructions for use are contained in each module).
* Module A
Calculation of predicted immunoassay results as calibration check
* Module C
Four parameter curve fitting and determination of sample concentrations from optical density values
* Module D
For calculation of EIA response to proposed or actual internal standard mixtures
 
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