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1.
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Place the anti-Dioxin antibody coated tubes
in rack and label. |
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2.
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Rinse tubes once by filling each tube with reagent
grade or bottled distilled water. Dump water out and tap inverted
tubes on absorbent material to remove excess water. |
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3.
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Add 0.5 mL of reagent grade or bottled distilled
water to each tube using Repeater Pipettor.
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4.
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Add 50 µL of 100 ppm Triton X-100 in methanol
to each tube and mix.
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5.
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Add 10 µL of negative control, standard or prepared
sample directly to liquid in each tube. Mix and let stand
2 hours (acceptable performance) or overnight (12 to 24
hours; best performance).
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6.
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Remove tube contents and wash 4 times using
reagent grade or bottled distilled water plus 0.01% Triton
X-100, 1.0 mL per tube for each wash. Tap inverted tubes on
absorbent material to remove excess water.
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7.
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Add 0.5 mL of Competitor-HRP Conjugate to each
tube using Repeater Pipettor. Mix and let stand 15 minutes.
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8.
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Remove tube contents and wash 4 times using
1.0 mL reagent grade or bottled distilled water per tube for
each wash. Tap inverted tubes on absorbent material to remove
excess water.
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9.
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Add 0.5 mL of HRP Substrate to each tube using
Repeater Pipettor. Mix and let stand 30 minutes.
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10.
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Add 0.5 mL of Stop to each tube using Repeater
Pipettor. Wipe outside dry and read OD within 30 minutes.
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