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1.
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Portable Differential
Photometer (preferred) |
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This is a portable dedicated tube reader designed specifically
for both the reading wavelength (450 nm, interference filter)
and the tube size (12x75 mm) used in this kit. Tubes are
read directly, removing the need to transfer liquids to
a cuvet or microplate for reading. Output is an LCD display;
data must be recorded by hand. Power is supplied by an on-board
NiCd battery; an AC adapter is included (both 110 and 220
V available). This reader can be used all day on battery
power after charging overnight. Most development work
on this kit was performed using this reader.
Source: Artel, Inc.,
Westbrook ME
Model: DP
Cost: approximately
$1000
Contact: Pat Frechette,
207-854-0860; fax 207-854-0867; email post@artel-usa.com
Note 1: Delivery will
take 1-3 weeks after placing an order (call to inquire).
Note 2: A modification
of this instrument is available with an RS-232 interface,
but at a higher cost and longer delivery time.
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1A.
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Benchtop Photometer
(alternative to 1) |
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This is a more sophisticated tube reader which can be purchased
already set up for both the reading wavelength (450 nm)
and the tube size (12x75 mm) used in this kit.
Tubes are read directly, removing the need to transfer liquids
to a cuvet or microplate for reading.
Output is a built-in paper tape printer
Includes a basic on-board software package, including data
reduction software, basis curve fitting, curve plotting,
and basic statistics.
Source: Source Scientific,
Garden Grove CA
Model: Microchem
Cost: approximately
$3300
Contact: Paul Gleason
800-888-9285 (USA) or 714-898-9001
Note 1: Delivery may
take up to 8 weeks after placing an order (call to inquire).
Note 2: Can be purchased
as either 110V or 220V.
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2.
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Positive Displacement
Micropipettor (preferred) |
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Adjustable micropipettor with disposable glass capillaries
for 1 to 10 µL volumes. The volume required for the
immunoassay is 10 µL of standards and samples.
This model has a highly polished stainless steel shaft
and is easily cleaned. Any pipettor which can deliver accurate
and precise 10 µL aliquots can be used, but ease of
cleaning and the potential for sample carryover must also
be carefully considered.
It is essential that only inert surfaces, such as glass,
stainless steel, or Teflon, contact the sample. Even plastics
which might give acceptable results at higher dioxin concentrations
must be avoided here. At the low analyte concentrations
used, even a small loss may represent a significant fraction
of the sample.
Source: Fisher Scientific
(Manufactured by Drummond Scientific as Dialamatic Microdispenser)
Model: Drummond # 3-000-210,
Fisher # 21-170-15A
Cost: Fisher list price
$95
Note 1: uses disposable
capillaries Drummond # 3-000-210G or Fisher # 21-169A, $11.00
per 100
Hamilton syringes can also be used, but require more extensive
rinsing than the recommended pipettor to avoid cross-contamination.
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3.
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Eppendorf
Repeater Plus Pipettor (preferred) |
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This is an adjustable positive displacement repeating pipettor
which uses disposable polypropylene tips. Delivery varies
from 1 µL to 10 mL per stroke, depending on tip and
pipettor setting. The tips used in the immunoassay are the
50 mL size for delivering 1 mL/tube wash solution and the
10 mL size for delivering 0.5 mL/tube of all other reagents.
This pipettor automatically senses and displays the volume
to be delivered. When tips or volume setting are changed,
the pipettor displays the new volume.
Source: Fisher Scientific
(Manufactured by Eppendorf for Brinkmann, distributed by
Fisher and others)
Model: Eppendorf Repeater
Plus, Brinkmann # 22-26-02-01, Fisher # 21-380-9
Cost: Fisher list price
$390
Note 1: The variety
pack of polypropylene tips includes 5 each of 8 sizes (Brinkmann
# 22-26-66-16, Fisher # 21-381-333) and costs $52.00 per
pack. Although these tips are intended to be disposable,
they can actually be reused many times before their performance
deteriorates.
Note 2: To use the 50
mL tips, it is necessary to also have a non-disposable adapter.
One of these is supplied in the variety pack in Note 1.
Extras (Brinkmann # 22-26-67-05, Fisher # NC-9365404) can
be bought for $13; at least one extra is recommended.
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3A.
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Other Positive
Displacement Repeating Pipettors
(alternative to 3) |
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Numerous other companies manufacture and sell similar repeating
pipettors. The volumes which are most frequently used in
the immunoassay are 0.5 mL and 1 mL per stroke. Any pipettor
which can repeatedly deliver accurate and precise aliquots
of 0.5 and 1.0 mL can be used.
Source: Various distributors
Model: Eppendorf Repeater,
Oxford, LabSystems, others
Cost: list prices approximately
$200 to $400
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