Two linked reactions form the basis of this type of test- the capture
by the antibody of an enzyme labeled dioxin fragment and the inhibition
of that capture by analyte molecules in the sample.
The baseline binding of the dioxin-enzyme conjugate to the antibody
is affected by both concentration and crossreactivity of analyte.
When dioxin-specific binding sites on the antibody are occupied by
analyte molecules, fewer sites are available to capture the dioxin-enzyme
Crossreactivity, the ability of an analyte molecule to bind to the
antibody, decreases as the structural similarity to the target analyte
Key concept: test response is by competitive inhibition and
is strongest for analyte structures which are most similar to the